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Survival of W. coagulans ATCC 7050, MA42, P13, and S5 under simulated gastrointestinal conditions. Black and white arrows indicate the addition of simulated gastric juice and simulated duodenal juice, respectively.

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Survival of W. coagulans ATCC 7050, MA42, P13, and S5 under simulated gastrointestinal conditions. Black and white arrows indicate the addition of simulated gastric juice and simulated duodenal juice, respectively.

Article Snippet: At the end of the treatments, statistical analysis of bacterial viability showed that the survival of the MA42, P13, and S5 strains was equivalent to that of the ATCC 7050 probiotic strain [ ].

Techniques:

Auto-aggregation ( A ) and cell surface hydrophobicity ( B ) of W. coagulans ATCC 7050, MA42, P13, and S5. Different letters (A–C) indicate significant differences in the values ( p < 0.05).

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Auto-aggregation ( A ) and cell surface hydrophobicity ( B ) of W. coagulans ATCC 7050, MA42, P13, and S5. Different letters (A–C) indicate significant differences in the values ( p < 0.05).

Article Snippet: At the end of the treatments, statistical analysis of bacterial viability showed that the survival of the MA42, P13, and S5 strains was equivalent to that of the ATCC 7050 probiotic strain [ ].

Techniques: Cell Surface Hydrophobicity

Disc diffusion assay of cell-free culture supernatants (CFCSs), obtained from W. coagulans ATCC 7050, W. coagulans MA42, W. coagulans S5, and W. coagulans P13 cultured in mMRS broth at 37 °C under static conditions for 24 h, against E. coli ATCC 25922, S. Typhimurium TISTR 292, B. cereus TISTR 747, and S. aureus TISTR 746. Control (M): uninoculated mMRS broth. Un-neutralized CFCS ( A ) and neutralized CFCS (pH 7) ( B ).

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Disc diffusion assay of cell-free culture supernatants (CFCSs), obtained from W. coagulans ATCC 7050, W. coagulans MA42, W. coagulans S5, and W. coagulans P13 cultured in mMRS broth at 37 °C under static conditions for 24 h, against E. coli ATCC 25922, S. Typhimurium TISTR 292, B. cereus TISTR 747, and S. aureus TISTR 746. Control (M): uninoculated mMRS broth. Un-neutralized CFCS ( A ) and neutralized CFCS (pH 7) ( B ).

Article Snippet: At the end of the treatments, statistical analysis of bacterial viability showed that the survival of the MA42, P13, and S5 strains was equivalent to that of the ATCC 7050 probiotic strain [ ].

Techniques: Diffusion-based Assay, Cell Culture, Control

Hemolytic activity of W. coagulans ATCC 7050, MA42, P13, and S5 on blood agar at 37 °C for 48 h. Staphylococcus aureus was used as the positive control.

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Hemolytic activity of W. coagulans ATCC 7050, MA42, P13, and S5 on blood agar at 37 °C for 48 h. Staphylococcus aureus was used as the positive control.

Article Snippet: At the end of the treatments, statistical analysis of bacterial viability showed that the survival of the MA42, P13, and S5 strains was equivalent to that of the ATCC 7050 probiotic strain [ ].

Techniques: Activity Assay, Positive Control

Viable cell count of W. coagulans MA42 and ATCC 7050 on modified MRS medium supplemented with glucose ( A ), wheat flour ( B ), oatmeal ( C ), xylan ( D ), carboxymethyl cellulose (CMC) ( E ), locust bean gum (LBG) ( F ), xanthan gum ( G ), pectin ( H ), inulin ( I ), xylo-oligosaccharides (XOS) ( J ), fructo-oligosaccharides (FOS) ( K ), and galacto-oligosaccharides (GOS) ( L ) as the sole carbon source. *, **, and *** indicate significant differences at p < 0.05, p < 0.01, and p < 0.001, respectively.

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Viable cell count of W. coagulans MA42 and ATCC 7050 on modified MRS medium supplemented with glucose ( A ), wheat flour ( B ), oatmeal ( C ), xylan ( D ), carboxymethyl cellulose (CMC) ( E ), locust bean gum (LBG) ( F ), xanthan gum ( G ), pectin ( H ), inulin ( I ), xylo-oligosaccharides (XOS) ( J ), fructo-oligosaccharides (FOS) ( K ), and galacto-oligosaccharides (GOS) ( L ) as the sole carbon source. *, **, and *** indicate significant differences at p < 0.05, p < 0.01, and p < 0.001, respectively.

Article Snippet: At the end of the treatments, statistical analysis of bacterial viability showed that the survival of the MA42, P13, and S5 strains was equivalent to that of the ATCC 7050 probiotic strain [ ].

Techniques: Cell Characterization, Modification

Enzyme activity profile of W. coagulans MA42 and W. coagulans ATCC 7050 cultivated in modified MRS medium supplemented with wheat flour ( A ), oatmeal ( B ), xylan ( C ), carboxymethyl cellulose (CMC) ( D ), locust bean gum (LBG) ( E ), xanthan gum ( F ), pectin ( G ), and inulin ( H ) as the sole carbon source at 37 °C for 48 h. * and ** indicate significant differences at p < 0.05 and p < 0.01, respectively.

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Enzyme activity profile of W. coagulans MA42 and W. coagulans ATCC 7050 cultivated in modified MRS medium supplemented with wheat flour ( A ), oatmeal ( B ), xylan ( C ), carboxymethyl cellulose (CMC) ( D ), locust bean gum (LBG) ( E ), xanthan gum ( F ), pectin ( G ), and inulin ( H ) as the sole carbon source at 37 °C for 48 h. * and ** indicate significant differences at p < 0.05 and p < 0.01, respectively.

Article Snippet: At the end of the treatments, statistical analysis of bacterial viability showed that the survival of the MA42, P13, and S5 strains was equivalent to that of the ATCC 7050 probiotic strain [ ].

Techniques: Activity Assay, Modification